In vitro antimicrobial activity of a new ophthalmic solution containing povidone-iodine 0.6% (IODIM® )

Abstract

Purpose: To assess the in vitro antimicrobial activity of a new commercial ophthalmic solution containing povidone-iodine 0.6% (IODIM^® ).

Methods: Staphylococcus aureus ATCC 43300, Pseudomonas aeruginosa ATCC 27853, three ocular bacterial isolates (1 S. epidermidis, 1 S. aureus, 1 P. aeruginosa) and five Candida species were used. The bacterial and fungal isolates were cultured on Columbia blood agar base plates and Sabouraud-dextrose agar plates, respectively and incubated overnight at 37°C. Bacterial and fungal suspensions in sterile saline solution were prepared to an optical density equal to 0.5 McFarland standard (approximately 10⁸ CFU/ml). Suspensions of the isolates were made in IODIM^® solution to obtain a final concentration of 10⁶ CFU/ml. The suspensions were then distributed in conical tubes in a final volume of 1 ml and incubated at 37°C. At different time-points (1, 5, 10, 15, 20, 25, 30 min and 24 hr), 10 μl of each suspension was removed, seeded on Columbia blood agar base and Sabouraud-dextrose agar plates and then incubated for 24 hr at 37°C. Positive and negative controls were included in all experiments.

Results: After 5-min incubation, there was no bacterial growth on any plate. Conversely, IODIM^® failed to kill the Candida isolates after 30 min' exposure and needed 24 hr to eradicate the organisms.

Conclusion: IODIM^® ophthalmic solution showed in vitro antimicrobial activity against S. epidermidis, S. aureus, P. aeruginosa and Candida species. Results suggest that it may be a potential candidate for the treatment of ocular surface infections and antimicrobial prophylaxis before intravitreal injections.

Keywords: Pseudomonas aeruginosa; Staphylococcus aureus; Staphylococcus epidermidis; Candida species; in vitro antimicrobial activity; povidone-iodine 0.6%.