Assessing the forensic efficiency of the GlobalFiler STR loci among the genetically isolated Chechen subpopulation in Jordan

Laith N Al-Eitan; Nizar N Darwish; Nancy M Hakooz; Rana B Dajani

doi:10.1016/j.gene.2019.144078

Assessing the forensic efficiency of the GlobalFiler STR loci among the genetically isolated Chechen subpopulation in Jordan

Gene. 2019 Dec 15:720:144078. doi: 10.1016/j.gene.2019.144078. Epub 2019 Aug 29.

Authors

Laith N Al-Eitan¹, Nizar N Darwish², Nancy M Hakooz³, Rana B Dajani⁴

Affiliations

¹ Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan; Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, Irbid 22110, Jordan. Electronic address: lneitan@just.edu.jo.
² Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid 22110, Jordan.
³ Department of Biopharmaceutics and Clinical Pharmacy, School of Pharmacy, University of Jordan, Amman 11942, Jordan.
⁴ Department of Biology and Biotechnology, Hashemite University, Zarqa 13133, Jordan; Radcliffe Institute for Advanced Studies, Harvard University, Cambridge, 02138, MA, USA.

PMID: 31473321
DOI: 10.1016/j.gene.2019.144078

Abstract

Short tandem repeats (STRs) are a widely utilized tool in forensic applications, the latter of which range from human identification and paternity testing to population analysis. The GlobalFiler STR loci, which includes 21 autosomal STRS, were analyzed in the Chechen subpopulation of Jordan. Whole blood samples were withdrawn from 159 Jordanian Chechen individuals, and genomic DNA was extracted from each sample. The GlobalFiler™ kit PCR Amplification Kit amplified and analyzed the STR loci on the 3130xl Genetic Analyzer using GeneMapper ID-X software. The combined match probability for the 21 autosomal STR loci was calculated to be 1.06 × 10^-24, a number that is highly discriminatory and informative. The SE33 (0.983) and TPOX (0.806) loci exhibited the highest and lowest powers of discrimination, respectively. Conclusively, the current study indicates that the GlobalFiler loci have a high utility in the Jordanian Chechen population, possibly paving the way for the future establishment of a reference population database in Jordan.

Keywords: Chechen; GlobalFiler; Jordan; STR.

MeSH terms

DNA / analysis*
DNA / genetics*
DNA Fingerprinting
Ethnicity / genetics*
Female
Forensic Genetics / statistics & numerical data*
Gene Frequency
Genetic Loci
Genetics, Population*
Humans
Male
Microsatellite Repeats*
Polymorphism, Genetic*

Substances

DNA