Structural characterization of transfection nanosystems based on tricationic surfactants and short double stranded oligonucleotides

Biochem Biophys Res Commun. 2019 Oct 22;518(4):706-711. doi: 10.1016/j.bbrc.2019.08.114. Epub 2019 Aug 28.

Abstract

For several years cationic surfactants have been the subjects of extensive studies as potential transgene carriers to be used in gene therapy. We report the formation of stable complexes between 21 base pairs oligonucleotides - siRNA, enhancing DMPK gene, and dsDNA and two tricationic surfactants (1,2,3-propanetri[oxymethyl-3-(1-dodecylimidazolium)]chloride and 1,2,3-propanetri[(oxymethyl)dimethyldodecylammonium]chloride. Structural studies by SAXS and TEM have shown that the dominant structure of the obtained lipoplexes is based on hexagonal, lamellar and cubic phases, packed in highly ordered aggregates. It has been established that tricationic surfactants can be used as siRNA carriers in gene therapy.

Keywords: DMPK; Gene therapy; SAXS; Tricationic surfactants; dsDNA; siRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cations / chemistry*
  • DNA / chemistry
  • DNA / genetics
  • Drug Carriers / chemistry*
  • Genetic Therapy / methods
  • Humans
  • Microscopy, Electron, Transmission
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Nanotechnology / methods*
  • Oligonucleotides / chemistry*
  • Oligonucleotides / genetics
  • RNA, Double-Stranded / chemistry
  • RNA, Double-Stranded / genetics
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • Scattering, Small Angle
  • Surface-Active Agents / chemistry*
  • Transfection
  • X-Ray Diffraction

Substances

  • Cations
  • Drug Carriers
  • Oligonucleotides
  • RNA, Double-Stranded
  • RNA, Small Interfering
  • Surface-Active Agents
  • DNA