Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

Deepak B Thimiri Govinda Raj; Niamat Ali Khan; Srisaran Venkatachalam; Sivakumar Arumugam; Selvamani Palaniswamy

doi:10.1007/7651_2019_254

Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNPs) for Efficient Coupling of Biomolecules

Methods Mol Biol. 2020:2125:73-75. doi: 10.1007/7651_2019_254.

Authors

Deepak B Thimiri Govinda Raj^{1

2}, Niamat Ali Khan^{3

4}, Srisaran Venkatachalam⁵, Sivakumar Arumugam⁶, Selvamani Palaniswamy⁷

Affiliations

¹ Envirotransgene® Bio-solutions Global, Chennai, India. deepak.balaji@bitsaa.org.
² DTCG®, Chennai, India. deepak.balaji@bitsaa.org.
³ Envirotransgene® Bio-solutions Global, Chennai, India.
⁴ DTCG®, Chennai, India.
⁵ Bannari Amman Institute of Technology, Sathyamangalam, Tamil Nadu, India.
⁶ School of Bio Sciences and Technology (SBST), VIT University, Vellore, Tamil Nadu, India.
⁷ Department of Pharmaceutical Technology, Anna University, Tiruchirappalli, Tamil Nadu, India.

PMID: 31463891
DOI: 10.1007/7651_2019_254

Abstract

Recently, we reported our methodology for isolating plasma membrane and lysosome from eukaryotic cell using superparamagnetic nanoparticles (SPMNPs). Here in this article, we report a step-by-step protocol for synthesis of hybrid gold nanoparticle (AuNP), surface functionalization of AuNPs on superparamagnetic nanoparticles (SPMNPs), and potential use of hybrid AuNP-SPMNP for efficient coupling of biomolecules.

Keywords: AuNPs; Bio-conjugation; Phospholipids; SPMNPs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dynamic Light Scattering
Gold / chemistry*
Magnetic Iron Oxide Nanoparticles / chemistry*
Magnetic Iron Oxide Nanoparticles / ultrastructure
Metal Nanoparticles / chemistry*

Substances

Gold