Label-Free Electrochemical Immunosensor Based on a Functionalized Ionic Liquid and Helical Carbon Nanotubes for the Determination of Cardiac Troponin I

Qihui Shen; Man Liu; Yang Lü; Dawei Zhang; Zhenyu Cheng; Yan Liu; Huajing Gao; Zhaohui Jin

doi:10.1021/acsomega.9b01152

Label-Free Electrochemical Immunosensor Based on a Functionalized Ionic Liquid and Helical Carbon Nanotubes for the Determination of Cardiac Troponin I

ACS Omega. 2019 Jul 9;4(7):11888-11892. doi: 10.1021/acsomega.9b01152. eCollection 2019 Jul 31.

Authors

Qihui Shen¹, Man Liu², Yang Lü¹, Dawei Zhang¹, Zhenyu Cheng¹, Yan Liu¹, Huajing Gao¹, Zhaohui Jin¹

Affiliations

¹ Department of Chemistry and Pharmaceutical Engineering and Department of Petrochemical Technology, Jilin Institute of Chemical Technology, Jilin 132022, P. R. China.
² State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012, P. R. China.

Abstract

A label-free electrochemical immunosensor for cardiac troponin I was prepared by using a helical carbon nanotube-supported aldehyde-functionalized ionic liquid. Because of the good conductivity of ionic liquid and helical carbon nanotubes, high sensitivity of the immunosensor was obtained. Functionalized ionic liquid provided binding sites for antibody, which simplified the process of sensor construction. Cardiac troponin I was detected by this immunosensor with a linear range of 0.05-30 ng/mL and a detection limit of 0.03 ng/mL. The electrochemical immunosensor had satisfactory reproducibility, high sensitivity, and acceptable specificity.