Design and Synthesis of Hybrid PEGylated Metal Monopicolinate Cyclam Ligands for Biomedical Applications

Fatima Aouidat; Zakaria Halime; Rosalba Moretta; Ilaria Rea; Stefania Filosa; Stella Donato; Rosarita Tatè; Luca de Stefano; Raphaël Tripier; Jolanda Spadavecchia

doi:10.1021/acsomega.8b03266

Design and Synthesis of Hybrid PEGylated Metal Monopicolinate Cyclam Ligands for Biomedical Applications

ACS Omega. 2019 Feb 1;4(2):2500-2509. doi: 10.1021/acsomega.8b03266. eCollection 2019 Feb 28.

Authors

Affiliations

¹ CNRS, UMR 7244, CSPBAT, Laboratoire de Chimie, Structures et Propriétés de Biomateriaux et d'Agents Therapeutiques Université Paris 13, 1 rue Chablis 93000, Sorbonne Paris Cité, 93000 Bobigny, France.
² Universitè de Brest, UMR-CNRS 6521/IBSAM, UFR Sciences et Techniques, 6 Avenue Victor le Gorgeu, C.S. 93837, 29238 Brest, France.
³ Institute for Microelectronics and Microsystems, Unit of Naples, CNR, Via P. Castellino 111, 80131 Naples, Italy.
⁴ Institute of Biosciences and Bioresources (IBBR), National Research Council (CNR), Naples, Italy-IRCCS, Neuromed, Via Università, 133, 80055 Pozzilli, Isernia, Italy.
⁵ Institute of Genetics and Biophysics "Adriano Buzzati-Traverso", CNR, Via P. Castellino 111, 80131 Naples, Italy.

Abstract

In this study, we report, for the first time, the synthesis of two original nanosystems, based on gold Au(III) and copper Cu(II): simple gold-copper nanoparticles (Cu⁰AuNPs) and enriched monopicolinate cyclam (L1)-Cu(II)-Au(III)-complex (L1@Cu²⁺AuNPs). The two nanomaterials differ substantially by the chelation or not of the Cu(II) ions during the NPs synthesis process. The two hybrid nanoparticles (Cu⁰AuNPs; L1@Cu²⁺AuNPs) were deeply studied from the chemical and physical point of view, using many different analytical techniques such as Raman and UV-vis spectroscopy, electron transmission microscopy, and dynamic light scattering. Both nanosystems show morphological and good chemical stability at pH 4 values and in physiological conditions during 98 h. Undifferentiated and neural differentiated murine embryonic stem cells were used as a model system for in vitro experiments to reveal the effects of NPs on these cells. The comparative study between Cu⁰AuNPs and L1@Cu²⁺AuNPs highlights that copper chelated in its +2 oxidation state in the NPs is more functional for biological application.