The effects of both graphene nanoplatelets and reduced graphene oxide as additives to the negative active material in valve-regulated lead-acid batteries for electric bikes were investigated. Low-temperature performance, charge acceptance, cycle performance, and water loss were investigated. The test results show that the low-temperature performance, charge acceptance, and large-current discharge performance of the batteries with graphene additives were significantly improved compared to the control battery, and the cycle life under 100% depth of discharge condition was extended by more than 52% from 250 to 380 cycles. Meanwhile, the amount of water loss from the batteries with graphene changed only slightly compared with the control cells. The excellent performance of the batteries can be ascribed to the graphene promoting the negative-plate charge and discharge processes and suppressing the growth of lead sulfate crystals.