Dyella amyloliquefaciens sp. nov., isolated from forest soil

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, rod-shaped and motile with single polar flagellum bacterial strain, designated DHC06^T, was isolated from forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, PR China. The strain grew at 4-37 °C (optimum, 28 °C), pH 4.5-8.0 (pH 6.0-7.5) and in the presence of 0-4.0 % (2.0 %, w/v) NaCl. In the 16S rRNA gene sequence phylogram, strain DHC06^T formed a clade with Dyella solisilvae DHG54^T and Dyella terrae KACC 12748^T within the genus of Dyella. Strain DHC06^T had 16S rRNA gene sequence similarities of 98.6, 98.3, 98.3 and 98.2 % to Dyella japonica DSM 16301^T, Dyella terrae JS14-6^T, Dyella soli KACC 12747^T and Dyella solisilvae DHG54^T, respectively. The distinctiveness of strain DHC06^Tfrom all described Dyellaspecies was also supported by the results of phylogenomic analysis based on 92 single-copy gene sequences. The DDH values among strain DHC06^T and closely related Dyella species were all lower than 70 %. Strain DHC06^T contained Q-8 as the only respiratory quinone. Its main fatty acids were iso-C_{15 : 0}, iso-C_{17 : 1} ω9c and summed feature 3 (C_{16 : 1} ω6c/C_{16 : 1} ω7c). The DNA G+C content of strain DHC06^T was 64.6 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of phenotypic, 16S rRNA gene sequence and genomic analyses and chemotaxonomic data, strain DHC06^T represents a novel species of the genus Dyella, for which the name Dyella amyloliquefaciens sp. nov. (type strain DHC06^T=GDMCC 1.1186^T=LMG 30090^T) is proposed.

Keywords: Dyella; Phylogeny; amyloliquefaciens; genome.