An efficient auxin-inducible degron system with low basal degradation in human cells

Nat Methods. 2019 Sep;16(9):866-869. doi: 10.1038/s41592-019-0512-x. Epub 2019 Aug 26.

Abstract

Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins / metabolism*
  • Carcinoma, Squamous Cell / drug therapy
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology
  • Cholesterol / metabolism
  • Cytoplasm / metabolism
  • HEK293 Cells
  • Humans
  • Indoleacetic Acids / pharmacology*
  • Plant Growth Regulators / pharmacology
  • Proteolysis / drug effects*
  • Receptors, Cell Surface / metabolism*
  • Skin Neoplasms / drug therapy
  • Skin Neoplasms / metabolism*
  • Skin Neoplasms / pathology
  • Transcription Factors / metabolism*
  • Tumor Cells, Cultured

Substances

  • AFB2 protein, Arabidopsis
  • Arabidopsis Proteins
  • IAA7 protein, Arabidopsis
  • Indoleacetic Acids
  • Plant Growth Regulators
  • Receptors, Cell Surface
  • Transcription Factors
  • Cholesterol