Novel sonochemical synthesis of Fe3O4 nanospheres decorated on highly active reduced graphene oxide nanosheets for sensitive detection of uric acid in biological samples

Balasubramanian Sriram; Mani Govindasamy; Sea-Fue Wang; R Jothi Ramalingam; Hamad Al-Lohedan; T Maiyalagan

doi:10.1016/j.ultsonch.2019.104618

Novel sonochemical synthesis of Fe₃O₄ nanospheres decorated on highly active reduced graphene oxide nanosheets for sensitive detection of uric acid in biological samples

Ultrason Sonochem. 2019 Nov:58:104618. doi: 10.1016/j.ultsonch.2019.104618. Epub 2019 May 29.

Authors

Balasubramanian Sriram¹, Mani Govindasamy¹, Sea-Fue Wang², R Jothi Ramalingam³, Hamad Al-Lohedan⁴, T Maiyalagan⁵

Affiliations

¹ Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan.
² Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan. Electronic address: sfwang@ntut.edu.tw.
³ Surfactant Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box-2455, Riyadh 11451, Saudi Arabia. Electronic address: jrajabathar@ksu.edu.sa.
⁴ Surfactant Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box-2455, Riyadh 11451, Saudi Arabia.
⁵ Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur, 603203 Chennai, Tamil Nadu, India. Electronic address: maiyalagan@gmail.com.

PMID: 31450380
DOI: 10.1016/j.ultsonch.2019.104618

Abstract

In this study, a super-active Iron (II, III) oxide nanospheres (Fe₃O₄ NPs) decorated reduced graphene oxide (rGOS) nanocomposite was developed. Fe₃O₄ NPs were stabilized on rGOS through electrostatic interactions in the aqueous medium. This process involves an ultrasound assisted reduction reaction of the GOS. The as-synthesized Fe₃O₄ NPs@rGOS was characterized through the HRTEM, SEM, XRD, Raman, elemental mapping and EDX analysis. The Fe₃O₄ NPs@rGOS modified GCE was developed for the determination of biomarker. Uric acid is important biomarker based on gout and kidney stone with high adverse effect in human body. The results obtained showed that the modified electrode Fe₃O₄ NPs@rGOS shows good electrochemical reduction peak compared to bare electrode and control electrodes. The Fe₃O₄ NPs@rGOS modified sensor linear range 0.02-783.6 µM was observed with nanomolar LOD 0.12 nM. In addition, the modified Fe₃O₄ NPs@rGOS/GCE sensor has been applied to determination of uric acid concentration in urine and blood serum samples. Furthermore, advantages of the modified sensor are high stability, repeatability and reproducibility.

Keywords: Biological samples; Iron oxide; Reduced graphene oxide; Sonochemical synthesis; Uric acid detection.

MeSH terms

Chemistry Techniques, Synthetic
Clinical Chemistry Tests / instrumentation
Clinical Chemistry Tests / methods*
Electrochemistry
Graphite / chemistry*
Humans
Hydrogen-Ion Concentration
Limit of Detection*
Magnetite Nanoparticles / chemistry*
Nanotechnology
Oxidation-Reduction
Oxides / chemistry
Ultrasonic Waves*
Uric Acid / blood*
Uric Acid / chemistry
Uric Acid / urine*

Substances

Magnetite Nanoparticles
Oxides
Uric Acid
Graphite