A novel electrochemical sensor for determination of DNA damage biomarker (8-hydroxy-2'-deoxyguanosine) in urine using sonochemically derived graphene oxide sheets covered zinc oxide flower modified electrode

Mani Govindasamy; Sea-Fue Wang; Bowya Subramanian; R Jothi Ramalingam; Hamad Al-Lohedan; Anandraj Sathiyan

doi:10.1016/j.ultsonch.2019.104622

A novel electrochemical sensor for determination of DNA damage biomarker (8-hydroxy-2'-deoxyguanosine) in urine using sonochemically derived graphene oxide sheets covered zinc oxide flower modified electrode

Ultrason Sonochem. 2019 Nov:58:104622. doi: 10.1016/j.ultsonch.2019.104622. Epub 2019 May 31.

Authors

Mani Govindasamy¹, Sea-Fue Wang², Bowya Subramanian³, R Jothi Ramalingam⁴, Hamad Al-Lohedan⁵, Anandraj Sathiyan⁶

Affiliations

¹ Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan.
² Department of Materials and Mineral Resources Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao East Rd., Taipei 106, Taiwan. Electronic address: sfwang@ntut.edu.tw.
³ Department of Electrical Engineering and Computer Science, National Taipei University of Technology, No.1, Section 3, Chung-Hsiao East Road, Taipei 106, Taiwan; Department of Information Technology, KSR College of Engineering, Tiruchengode, Tamil Nadu, India.
⁴ Surfactant Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box-2455, Riyadh 11451, Saudi Arabia. Electronic address: jrajabathar@ksu.edu.sa.
⁵ Surfactant Research Chair, Chemistry Department, College of Science, King Saud University, P.O. Box-2455, Riyadh 11451, Saudi Arabia.
⁶ Department of Chemistry, Bishop Heber College, Trichy 620017, Tamil Nadu, India.

PMID: 31450347
DOI: 10.1016/j.ultsonch.2019.104622

Abstract

To explore a novel and multi-layer based graphene oxide covered zinc oxide nanoflower (ZnO NFs@GOS) as a modified electrode materials by sonochemical technique (40 kHz, 300 W). Herein, novel nanocomposite is successfully characterized by various characterization analysis (FESEM, HRTEM, XRD, XPS and (EIS) electrochemical impedance spectroscopy) and employed as high sensitive modified electrode (ZnO NFs@GOS nanocomposite) for the electrochemical determination of biomarker. 8-hydroxy-2'-deoxyguanosine (8-HDG) is one of the important cancer and oxidative stress biomarker. The results demonstrated that the ZnO NFs@GOS modified SPCE reveal well-defined electro-oxidation peak at 0.36 V (vs. Ag/AgCl). The high sensitive properties of the optimized flower like modified electrode are because of the excellent synergistic effect of the ZnO flower and the graphene oxide nanosheets, as evidenced by a superior bio-sensing performance. The nanocomposite fabricated modified biosensor was facilitating the analysis of 8-HDG in the concentration ranges of 0.05-536.5 µM with a low detection limit is 8.67 nM. The ZnO NFs@GOS modified sensor can also employed for the determination of 8-HDG in human urine samples, promising its application towards the quantification of cancer biomarker in biological samples.

Keywords: DNA damage biomarker; Graphene oxide nanosheets; Sonochemical synthesis; Urine real sample; Zinc oxide nanoflower.

MeSH terms

8-Hydroxy-2'-Deoxyguanosine / chemistry
8-Hydroxy-2'-Deoxyguanosine / urine*
Biomarkers / chemistry
Biomarkers / urine
Catalysis
DNA Damage*
Electrochemistry / instrumentation*
Electrodes
Graphite / chemistry*
Limit of Detection
Ultrasonic Waves*
Urinalysis / instrumentation*
Zinc Oxide / chemistry*

Substances

Biomarkers
Graphite
8-Hydroxy-2'-Deoxyguanosine
Zinc Oxide