Molecular surveillance and genetic analyses of bufavirus in environmental water in Thailand

Nattika Nantachit; Pattara Khamrin; Kattareeya Kumthip; Rungnapa Malasao; Niwat Maneekarn

doi:10.1016/j.meegid.2019.104013

Molecular surveillance and genetic analyses of bufavirus in environmental water in Thailand

Infect Genet Evol. 2019 Nov:75:104013. doi: 10.1016/j.meegid.2019.104013. Epub 2019 Aug 22.

Authors

Nattika Nantachit¹, Pattara Khamrin¹, Kattareeya Kumthip¹, Rungnapa Malasao², Niwat Maneekarn³

Affiliations

¹ Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand.
² Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand; Department of Community Medicine, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
³ Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand. Electronic address: niwat.m@cmu.ac.th.

PMID: 31446136
DOI: 10.1016/j.meegid.2019.104013

Abstract

Bufavirus (BuV), a recent discovery virus of the family Parvoviridae, has been suggested to be a potential causative agent of diarrhea in humans. The prevalence of BuV has been demonstrated mostly in fecal specimens of diarrheic patients. Little is known about the presence of BuV in the environmental water. The aim of the present study was to conduct a surveillance for BuV in the environmental water in Thailand. A total of 125 water samples were collected during November 2016 and July 2018 from six different areas in Chiang Mai city, Thailand. Water samples were concentrated and extracted to obtain BuV genomic DNA. The BuV was screened by amplification of NS1 region using nested-PCR. The detected BuV was further characterized by amplification of VP2 region. The NS1 and VP2 genes were sequenced and analyzed phylogenetically. The BuV strain (CMW88/18) detected in this study belonged to BuV1 with the prevalence of 0.8%. The CMW88/18 strain was most closely related to human BuV1 strains reported previously worldwide suggesting contamination of BuV in the environmental water could be a potential source of infection in human.

Keywords: Bufavirus; Diarrhea; Environmental water; Gastroenteritis; Genotype 1; Thailand.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diarrhea / epidemiology
Diarrhea / virology
Genome, Viral*
Genomics* / methods
Humans
Parvoviridae / classification*
Parvoviridae / genetics*
Parvoviridae Infections / epidemiology
Parvoviridae Infections / virology
Phylogeny
Public Health Surveillance
Thailand / epidemiology
Water Microbiology*