Effect of different zinc concentrations on partially-stabilized cement for vital pulp therapy

Chih-Chun Chang; Chun-Liang Yeh; Hao-Hueng Chang; Yi-Fang Kuo; Po-Ya Huang; Chun-Pin Lin

doi:10.1016/j.jfma.2019.08.005

Effect of different zinc concentrations on partially-stabilized cement for vital pulp therapy

J Formos Med Assoc. 2019 Dec;118(12):1610-1615. doi: 10.1016/j.jfma.2019.08.005. Epub 2019 Aug 21.

Authors

Chih-Chun Chang¹, Chun-Liang Yeh¹, Hao-Hueng Chang², Yi-Fang Kuo¹, Po-Ya Huang¹, Chun-Pin Lin³

Affiliations

¹ Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
² Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan.
³ Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: chunpinlin@gmail.com.

PMID: 31445847
DOI: 10.1016/j.jfma.2019.08.005

Abstract

Background/purpose: We have developed and investigated the partially-stabilized cements (PSC) with Zn for vital pulp therapy due to their short setting time and high cell biocompatibility. However, the effect of PSC with different concentrations of Zn on setting time and biocompatibility remained unknown. Therefore, the purpose of this study was to determine the optimal concentration of Zn to be synthesized with PSC for vital pulp therapy.

Methods: PSC with different weight percentages of Zn (5%, 7%, 10%) were synthesized to attain 5%Zn-PSC, 7%Zn-PSC, and 10%Zn-PSC. The initial and final setting times were measured using the Gillmore needles method, and the compressive strength tests were conducted using a universal testing machine. The phases of Zn-PSC powders were observed using an X-ray diffractometer (XRD). Human dental pulp stem cells (hDPSCs) were used to evaluate the biocompatibility and cytotoxicity of the materials via Alamar blue and LDH assays. Mineral trioxide aggregate (MTA) was used to be compared with Zn-PSC samples.

Results: The initial and final setting times of PSC with different concentrations of Zn were reduced considerably compared to those of MTA. The results also indicated that the initial and final setting times decreased as the weight % of Zn increased. 5%Zn-PSC had the highest compressive strength among all tested materials. 5%Zn-PSC samples also displayed comparatively higher cell biocompatibility than 7% and 10% Zn-PSC samples. However, there was no significant difference between the 5%Zn-PSC and MTA in cell biocompatibility. In addition, the results of the LDH release assay indicated a low level of cytotoxicity among all the test samples.

Conclusion: 5%Zn-PSC has a shorter setting time, better mechanical properties, and good biocompatibility and thus it has great potential for vital pulp therapy.

Keywords: Biocompatibility; Partially-stabilized cement; Setting time; Vital pulp therapy; Zinc.

MeSH terms

Aluminum Compounds
Biocompatible Materials
Calcium Compounds
Cell Survival / drug effects
Cells, Cultured
Dental Cements*
Dental Pulp / cytology*
Dental Pulp Capping*
Drug Combinations
Humans
Materials Testing
Oxides
Silicates
Stem Cells / drug effects*
Zinc / chemistry
Zinc / pharmacology*

Substances

Aluminum Compounds
Biocompatible Materials
Calcium Compounds
Dental Cements
Drug Combinations
Oxides
Silicates
mineral trioxide aggregate
Zinc