Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9

Hyeong-Jun Han; Hyang-Hee Seo; Hyo-Won Han; Jung-Hyun Kim

doi:10.1016/j.scr.2019.101520

Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9

Stem Cell Res. 2019 Oct:40:101520. doi: 10.1016/j.scr.2019.101520. Epub 2019 Jul 31.

Authors

Hyeong-Jun Han¹, Hyang-Hee Seo¹, Hyo-Won Han¹, Jung-Hyun Kim²

Affiliations

¹ Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
² Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea. Electronic address: kjhcorea@korea.kr.

PMID: 31445394
DOI: 10.1016/j.scr.2019.101520

Abstract

Toll Like Receptor (TLR) family plays an important role in the activation of innate immunity against pathogens. TLR7 mediates the recognition of single-stranded RNA viruses, such as human immunodeficiency virus, hepatitis C virus, and influenza virus in endosomes. Here, we generated a TLR7 homozygous knockout human induced pluripotent cell (hiPSC) line, hiPSC-TLR7KO-A59, using the CRISPR/Cas9 genome editing method. The hiPSC-TLR7KO-A59 line maintains normal morphology, pluripotency, and differentiation capacity into three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems / genetics*
Cell Line
Embryoid Bodies / cytology
Embryoid Bodies / metabolism
Gene Editing
Homozygote
Humans
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / transplantation
Karyotype
Male
Mice
Mice, Inbred NOD
Mice, SCID
Teratoma / pathology
Toll-Like Receptor 7 / genetics*

Substances

Toll-Like Receptor 7