Metabolites analysis for cold-resistant yeast (Wickerhamomyces anomalus) strains own antioxidant activity on cold stored fish mince

Abstract

The effect of eight cold-resistant yeast strains (J3, J7, J8, J9, J12, J15, J18, and J25) of Wickerhamomyces anomalus on the lipid oxidation of cold stored fish mince (4 °C) were investigated. And the metabolites of these yeast were determined with gas chromatography-mass spectrometry. These strains could effectively inhibit the increase of hydroperoxides value (p < 0.05), and the inhibiting rate was positively correlated with the content of isolongifolene, xylitol, turanose, thymol-glucoside, and uridine. Especially, the J3, J7, J8, J9, J12, and J18 could eliminate a large part of thiobarbituric acid reactive substances (TBARS) (p < 0.05), the eliminating rate was proportionate to the aldehyde dehydrogenase activity. Several bacteriostatic metabolites were detected: thymol-glucoside, 2-phenylethanol, cedro, and 2,4-bis (1,1-dimethylethyl) phenol. In addition, W. anomalus produced many metabolites with fruit and floral notes. In conclusion, cold-resistant W. anomalus strains own antioxidant activity were potential new bio-preservatives in the cold storage of muscle products.

Keywords: 2-phenylethanol (PubChem CID: 6054); Allose (PubChem CID: 439507); Antioxidant; Arabinose (PubChem CID: 5460291); Bio-preservative; Cedrol (PubChem CID: 65575); Cold-resistant; Dulcitol (PubChem CID: 11850); Erythritol (PubChem CID: 222285); Glycerol (PubChem CID: 753); Isolongifolene (PubChem CID: 11127402); Lipid oxidation; Mannobiose (PubChem CID: 152109); Metabolite; Threose (PubChem CID: 439665); Thymol-glucoside (PubChem CID: 88687); Turanose(PubChem CID: 5460935); Wickerhamomyces anomalus; Xylitol (PubChem CID: 6912); ridine(PubChem CID: 6029).