Polydatin prevents LPS-induced acute kidney injury through inhibiting inflammatory and oxidative responses

Abstract

The anti-inflammatory property of polydatin, a natural active ingredient found in the rhizome of Polygonum cuspidatum, has been verified. Although a variety of physiological functions have been uncovered, the protective effects and mechanism of polydatin on LPS-induced acute kidney injury remain unclear. Kidney histological change, MDA content, MPO activity, TNF-α, IL-1β, and IL-6 production were measured in this study. Furthermore, NF-κB and Nrf2 were tested by western blotting. In this study, polydatin not only significantly attenuated serum creatinine and BUN levels, but also remarkably inhibited TNF-α, IL-1β, and IL-6 production, MPO activity, and MDA content. Polydatin significantly inhibited LPS-induced NF-κB activation. Also, polydatin significantly increased Nrf2 and HO-1 expression. Taken together, all the above results indicate that polydatin had protective effects against LPS-induced AKI by blocking inflammatory and oxidative responses.

Keywords: Kidney injury; LPS; Nrf2; TNF-α.