Kinetics of lipid raft formation at lipid monolayer-bilayer junction probed by surface plasmon resonance

Yong-Sang Ryu; Hansik Yun; Taerin Chung; Jeng-Hun Suh; Sungho Kim; Kyookeun Lee; Nathan J Wittenberg; Sang-Hyun Oh; Byoungho Lee; Sin-Doo Lee

doi:10.1016/j.bios.2019.111568

Kinetics of lipid raft formation at lipid monolayer-bilayer junction probed by surface plasmon resonance

Biosens Bioelectron. 2019 Oct 1:142:111568. doi: 10.1016/j.bios.2019.111568. Epub 2019 Aug 10.

Authors

Affiliations

¹ School of Electrical and Computer Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea; Sensor System Research Center, Korea Institute of Science and Technology, Seongbuk-gu, Seoul, 02792, South Korea.
² School of Electrical and Computer Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
³ Inter-University Semiconductor Research Center, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea.
⁴ Department of Electrical and Computer Engineering, University of Minnesota, 200 Union St SE, Minneapolis, MN, 55455, USA; Department of Chemistry, Lehigh University, Bethlehem, PA, 18015, USA.
⁵ Department of Electrical and Computer Engineering, University of Minnesota, 200 Union St SE, Minneapolis, MN, 55455, USA.
⁶ School of Electrical and Computer Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea; Inter-University Semiconductor Research Center, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea. Electronic address: sidlee@plaza.snu.ac.kr.

PMID: 31442945
DOI: 10.1016/j.bios.2019.111568

Abstract

A label-free, non-dispruptive, and real-time analytical device to monitor the dynamic features of biomolecules and their interactions with neighboring molecules is an essential prerequisite for biochip- and diagonostic assays. To explore one of the central questions on the lipid-lipid interactions in the course of the liquid-ordered (l_o) domain formation, called rafts, we developed a method of reconstituting continuous but spatially heterogeneous lipid membrane platforms with molayer-bilayer juntions (MBJs) that enable to form the l_o domains in a spatiotemporally controlled manner. This allows us to detect the time-lapse dynamics of the lipid-lipid interactions during raft formation and resultant membrane phase changes together with the raft-associated receptor-ligand binding through the surface plasmon resonance (SPR). For cross-validation, using epifluorescence microscopy, we demonstrated the underlying mechanisms for raft formations that the infiltration of cholesterols into the sphingolipid-enriched domains plays a crucial roles in the membrane phase-separation. Our membrane platform, being capable of monitoring dynamic interactions among lipids and performing the systematic optical analysis, will unveil physiological roles of cholesterols in a variety of biological events.

Keywords: Lipid monolayer-bilayer junction; Lipid raft; Lipid-lipid interaction; Optical sensors; Real-time; Surface plasmon resonance.

MeSH terms

Animals
Cholesterol / analysis
Cholesterol / metabolism*
Equipment Design
Humans
Kinetics
Lab-On-A-Chip Devices*
Lipid Bilayers / analysis
Lipid Bilayers / metabolism*
Membrane Microdomains / chemistry
Membrane Microdomains / metabolism*
Models, Molecular
Phase Transition
Protein Binding
Surface Plasmon Resonance / instrumentation*
Surface Plasmon Resonance / methods

Substances

Lipid Bilayers
Cholesterol