Tools to detect human norovirus infectivity have been lacking. Using human intestinal enteroid cultures inoculated with GII.Pe-GII.4 Sydney-infected fecal samples, we determined that a real-time reverse transcription PCR cycle threshold cutoff of 30 may indicate infectious norovirus. This finding could be used to help guide infection control.
Keywords: Ct cutoff; Cycle threshold; Hong Kong; enteric infections; enteroids; human norovirus; infectivity; rRT-PCR; real-time reverse transcription PCR; viruses.