Multi-linear gradients are a convenient solution to get separation of complex samples by modulating carefully the gradient slope, in order to accomplish the local selectivity needs for each particular solute cluster. These gradients can be designed by trial-and-error according to the chromatographer experience, but this strategy becomes quickly inappropriate for complex separations. More evolved solutions imply the sequential construction of multi-segmented gradients. However, this strategy discards part of the search space in each step of the construction and, again, cannot deal properly with very complex samples. When the complexity is too large, the only valid alternative for finding the best gradient is the use of global search methods, such as genetic algorithms (GAs). Recently, a new global approach where the level of detail is increased along the search has been proposed, namely Multi-scale optimisation (MSO). In this strategy, cubic splines are applied to build intermediate curves to define any arbitrary solvent variation function. Subdivision schemes are used to generate the cubic splines and control their level of detail. The search was subjected to a number of restrictions, such as avoiding long elution and favouring a balanced peak distribution. The aim of this work is evaluating and comparing the results of GAs and MSO. Both approaches were tested with a set of 14 diuretics and probenecid, eluted with acetonitrile-water mixtures using a C18 column. Satisfactory baseline resolution was obtained with an analysis time of 15-16 min. We found that GAs optimisation offered results equivalent to those provided by MSO, when the penalisation parameters were included in the cost function.
Keywords: Diuretic compounds; Genetic algorithms; Interpretive optimisation; Multi-scale optimisation; Reversed-phase liquid chromatography.
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