Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types

Maria Luisa Menandro; Giorgia Dotto; Alessandra Mondin; Marco Martini; Letizia Ceglie; Daniela Pasotto

doi:10.1016/j.cimid.2019.101331

Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types

Comp Immunol Microbiol Infect Dis. 2019 Oct:66:101331. doi: 10.1016/j.cimid.2019.101331. Epub 2019 Jul 4.

Authors

Maria Luisa Menandro¹, Giorgia Dotto², Alessandra Mondin³, Marco Martini⁴, Letizia Ceglie⁵, Daniela Pasotto⁶

Affiliations

¹ Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: marialuisa.menandro@unipd.it.
² Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: giorgia.dotto@unipd.it.
³ Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: alessandra.mondin@unipd.it.
⁴ Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: marco.martini@unipd.it.
⁵ Istituto Zooprofilattico Sperimentale delle Venezie, Viale dell'Università, 10-35020, Legnaro, PD, Italy. Electronic address: lceglie@izsve.it.
⁶ Department of Animal Medicine, Productions and Health, University of Padua, Viale dell'Università, 16-35020, Legnaro, PD, Italy. Electronic address: daniela.pasotto@unipd.it.

PMID: 31437680
DOI: 10.1016/j.cimid.2019.101331

Abstract

The aim of this study was to assess the prevalence, the genotypic diversity, the antimicrobial resistance traits of canine and feline clinical methicillin-resistant Staphylococcus pseudintermedius (MRSP) isolates in a diagnostic laboratory in Italy during 2015-2016. All isolates were characterized by multilocus sequence typing (MLST), staphylococcal cassette chromosome (SCC)-mec typing and staphylococcal protein A (spa)-typing. The resistance profiles were assessed by antimicrobial susceptibility testing and confirmed genotypically by the detection of mecA gene and by microarray analyses. The prevalence of MRSP isolates was high (31.6%). All the strains were multidrug resistant and the most frequent clone was ST71-SCCmec type II-III. These results confirm a high prevalence of MRSP amongst clinical samples from pets in Italy. These isolates show multidrug resistance features that are of concern both in veterinary and human medicine for clinical and epidemiological reasons.

Keywords: Antimicrobial-resistance; Cat; Dog; Methicillin-resistant Staphylococcus pseudintermedius; Microarray; Multilocus sequence typing.

MeSH terms

Animals
Bacterial Typing Techniques
Cat Diseases / epidemiology*
Cat Diseases / microbiology
Cats
Dog Diseases / epidemiology*
Dog Diseases / microbiology
Dogs
Genetic Variation
Genotype
Italy / epidemiology
Methicillin / pharmacology
Methicillin Resistance*
Microbial Sensitivity Tests
Multilocus Sequence Typing
Pets / microbiology*
Prevalence
Staphylococcal Infections / epidemiology
Staphylococcal Infections / veterinary*
Staphylococcus / classification
Staphylococcus / drug effects*
Staphylococcus / genetics

Substances

Methicillin