Establishment of a simultaneous detection method for ten duck viruses using MALDI-TOF mass spectrometry

J Virol Methods. 2019 Nov:273:113723. doi: 10.1016/j.jviromet.2019.113723. Epub 2019 Aug 17.

Abstract

Rapid screening of infectious viral diseases is the key to ensure healthy development of duck livestock industry. Currently routine viral detection methods are primarily used to detect up to 3 viruses. In this study, matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) was used for simultaneous detection and genotyping of ten viruses in duck, including Duck hepatitis A virus 1 (DHAV-1), DHAV-3, Duck astrovirus 1 (DAstV-1), DAstV-2, Duck reovirus 1 (DRV-1), DRV-2, Tembusu virus (TMUV), Avian influenza virus (AIV), Goose parvovirus (GPV) and Duck enteritis virus (DEV). The low detection limits of this proposed method for ten duck viruses ranged from 1.3 copies/μl to 7.8 copies/μl. The novel detection method with high sensitivity, good specificity and high throughput has the potential to be applied for disease diagnosis and surveillance.

Keywords: Duck; MALDI-TOF; Mass spectrometry; Simultaneous detection; Viruses.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • DNA, Viral / isolation & purification
  • Ducks / virology*
  • Genotyping Techniques
  • Limit of Detection
  • Multiplex Polymerase Chain Reaction / methods
  • Poultry Diseases / virology*
  • RNA, Viral / isolation & purification
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Viruses / classification
  • Viruses / genetics
  • Viruses / isolation & purification*

Substances

  • DNA, Viral
  • RNA, Viral