A dual-signal strategy is proposed based on fluorescent biomass-based carbon dots (BC-dots) and chitosan stabilized AuNPs (CS@AuNPs) to determine hyaluronidase (HAase). BC-dots can induce aggregation of CS@AuNPs nanoparticles with a colour change from red to blue. Positively charged CS@AuNPs interacted with the negatively charged hyaluronic acid (HA) through electrostatic adsorption, and CS@AuNPs maintained stability due to the semirigid coil conformation of HA. However, in the presence of HAase, due to enzymatic hydrolysis of HA by HAase, the CS@AuNPs agglomerated. Based on the change of fluorescence and colour, quantitative analysis of HAase was achieved. Linear ranges for the fluorometric and colorimetric determinations were 2.0-70 U mL-1 and 8-60 U mL-1 , respectively, with a detection limit of 0.27 U mL-1 . This dual-signal sensing system possesses high potential for determination of HAase in biological matrices.
Keywords: biomass-based carbon dots; colorimetric; electrostatic adsorption; fluorometric; semirigid coil conformation.
© 2019 John Wiley & Sons, Ltd.