Genome Sequences and Methylation Patterns of Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, Two Extremely Halophilic Archaea from a Bolivian Salt Mine

Priya DasSarma; Brian P Anton; Satyajit L DasSarma; Fabiana L Martinez; Daniel Guzman; Richard J Roberts; Shiladitya DasSarma

doi:10.1128/MRA.00810-19

Genome Sequences and Methylation Patterns of Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, Two Extremely Halophilic Archaea from a Bolivian Salt Mine

Microbiol Resour Announc. 2019 Aug 15;8(33):e00810-19. doi: 10.1128/MRA.00810-19.

Authors

Priya DasSarma¹, Brian P Anton², Satyajit L DasSarma¹, Fabiana L Martinez^{1

3}, Daniel Guzman⁴, Richard J Roberts², Shiladitya DasSarma⁵

Affiliations

¹ Institute of Marine and Environmental Technology, Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, USA.
² New England BioLabs, Ipswich, Massachusetts, USA.
³ Instituto de Investigaciones para la Industria Química, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Salta, Salta, Argentina.
⁴ Centro de Biotecnología, Faculty of Sciences and Technology, Universidad Mayor de San Simón, Cochabamba, Bolivia.
⁵ Institute of Marine and Environmental Technology, Department of Microbiology and Immunology, University of Maryland, Baltimore, Maryland, USA sdassarma@som.umaryland.edu.

Abstract

Two extremely halophilic archaea, namely, Natrinema versiforme BOL5-4 and Natrinema pallidum BOL6-1, were isolated from a Bolivian salt mine and their genomes sequenced using single-molecule real-time sequencing. The GC-rich genomes of BOL5-4 and BOL6-1 were 4.6 and 3.8 Mbp, respectively, with large chromosomes and multiple megaplasmids. Genome annotation was incorporated into HaloWeb and methylation patterns incorporated into REBASE.