Gas-phase fragmentation pathways of host-guest complexes of cyclodextrins (CDs) and polyoxometalates (POMs) were examined using collision-induced dissociation (CID). The host-guest complexes studied here were composed of two different classes of POMs-Keggin (PW12O403-) and Lindqvist (M6O192-, M = Mo, W)-and three types of CDs (α-, β-, and γ-CD) differing in the diameter of the inner cavity. The CD-POM complexes were generated either by mixing methanol solutions of POM and CD or through a one-step acidic condensation of tetraoxometalates MO42- (M = Mo, W) with CDs for complexes with Keggin and Lindqvist anions, respectively, and introduced into the gas phase using electrospray ionization (ESI). We observe distinct differences in fragmentation pathways of the complexes of Keggin and Lindqvist POMs under high- and low-energy CID conditions. Specifically, direct dissociation and proton transfer from CD to POM accompanied by the separation of fragments is observed in CID of Keggin CD-POM complexes. In contrast, dissociation of CD complexes with Lindqvist POMs is dominated by the simultaneous loss of multiple water molecules. This unusual fragmentation channel is attributed to dissociation of the POM cluster inside the CD cavity accompanied by covalent bond formation between the fragments and CD and elimination of multiple water molecules. The observed covalent coupling of metal oxide clusters opens up opportunities for derivatization of macrocyclic host molecules using collisional excitation of gaseous non-covalent complexes.
Keywords: Collision-induced dissociation; Covalent coupling; Cyclodextrins; Host-guest chemistry; Mass spectrometry; Polyoxometalates.