Eradication of a Multidrug-Resistant, Carbapenemase-Producing Klebsiella pneumoniae Isolate Following Oral and Intra-rectal Therapy With a Custom Made, Lytic Bacteriophage Preparation

Mario Corbellino; Nicolas Kieffer; Mzia Kutateladze; Nana Balarjishvili; Lika Leshkasheli; Lia Askilashvili; George Tsertsvadze; Sara Giordana Rimoldi; Deia Nizharadze; Naomi Hoyle; Lia Nadareishvili; Spinello Antinori; Cristina Pagani; Daniele Giuseppe Scorza; Ai Ling Loredana Romanò; Sandro Ardizzone; Piergiorgio Danelli; Maria Rita Gismondo; Massimo Galli; Patrice Nordmann; Laurent Poirel

doi:10.1093/cid/ciz782

Eradication of a Multidrug-Resistant, Carbapenemase-Producing Klebsiella pneumoniae Isolate Following Oral and Intra-rectal Therapy With a Custom Made, Lytic Bacteriophage Preparation

Clin Infect Dis. 2020 Apr 15;70(9):1998-2001. doi: 10.1093/cid/ciz782.

Authors

Mario Corbellino¹, Nicolas Kieffer², Mzia Kutateladze³, Nana Balarjishvili³, Lika Leshkasheli³, Lia Askilashvili³, George Tsertsvadze³, Sara Giordana Rimoldi⁴, Deia Nizharadze⁵, Naomi Hoyle⁵, Lia Nadareishvili⁵, Spinello Antinori^{1

6}, Cristina Pagani⁴, Daniele Giuseppe Scorza⁴, Ai Ling Loredana Romanò⁷, Sandro Ardizzone^{6

8}, Piergiorgio Danelli^{6

9}, Maria Rita Gismondo^{4

6}, Massimo Galli^{1

6}, Patrice Nordmann², Laurent Poirel²

Affiliations

¹ III Division of Infectious Diseases, Azienda Socio Sanitaria Territoriale (ASST) Fatebenefratelli Sacco, Milano, Italy.
² Medical and Molecular Microbiology, Faculty of Science and Medicine, Institut National de la Santé et de la Recherche Médicale (INSERM) European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland.
³ G. Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi, Georgia.
⁴ Division of Microbiology, ASST Fatebenefratelli-Sacco, Milano, Italy.
⁵ Eliava Phage Therapy Center LLC, Tbilisi, Georgia.
⁶ Luigi Sacco Department of Clinical and Biomedical Sciences, University of Milano, Milano, Italy.
⁷ Division of Urology, ASST Fatebenefratelli - Sacco, Milano, Italy.
⁸ Division of Gastroenterology, ASST Fatebenefratelli - Sacco, Milano, Italy.
⁹ Division of General Surgery, ASST Fatebenefratelli - Sacco, Milano, Italy.

PMID: 31414123
DOI: 10.1093/cid/ciz782

Abstract

In July 2017, a patient presented colonization with a multidrug-resistant, carbapenemase (KPC-3)-producing Klebsiella pneumoniae isolate. A custom-made, lytic bacteriophage preparation was administered to the patient in December 2017, with subsequent eradication of the microorganism and without adverse effects.

Keywords: antibiotic resistance; bacteriophage; multidrug-resistant Klebsiella pneumoniae; personalized phage therapy; selective decolonization.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / therapeutic use
Bacterial Proteins
Bacteriophages*
Culture
Humans
Klebsiella Infections* / drug therapy
Klebsiella pneumoniae
beta-Lactamases

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
carbapenemase