Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues

Dapeng Peng; Owen Kavanagh; Haijiao Gao; Xiya Zhang; Sijun Deng; Dongmei Chen; Zhenli Liu; Changqing Xie; Chen Situ; Zonghui Yuan

doi:10.1016/j.foodchem.2019.04.022

Surface plasmon resonance biosensor for the determination of 3-methyl-quinoxaline-2-carboxylic acid, the marker residue of olaquindox, in swine tissues

Food Chem. 2020 Jan 1:302:124623. doi: 10.1016/j.foodchem.2019.04.022. Epub 2019 Apr 6.

Authors

Affiliations

¹ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
² School of Health Sciences, Lord Mayor's Walk, York Y031 7EX, UK.
³ The Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, BT9 5AG Northern Ireland, UK.
⁴ The Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, BT9 5AG Northern Ireland, UK. Electronic address: c.situ@qub.ac.uk.
⁵ National Reference Laboratory of Veterinary Drug Residues (HZAU) and MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: yuan5802@mail.hzau.edu.cn.

PMID: 31408774
DOI: 10.1016/j.foodchem.2019.04.022

Abstract

To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 µg kg^-1 in swine muscle and 2.7 µg kg^-1 in swine liver, which are lower than the EU recommended concentration (10 µg kg^-1). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.

Keywords: 3-Methyl-quinoxaline-2-carboxylic acid; Olaquindox; Residues; Surface plasmon resonance biosensor; Swine tissues.

MeSH terms

Animals
Drug Residues / metabolism*
Limit of Detection
Liver / chemistry
Muscles / chemistry
Quinoxalines / analysis*
Quinoxalines / metabolism*
Reproducibility of Results
Surface Plasmon Resonance*
Swine

Substances

3-methylquinoxaline-2-carboxylic acid
Quinoxalines
olaquindox