miRNAs induced by white spot syndrome virus involve in immunity pathways in shrimp Litopenaeus vannamei

Wenlin Wu; Congjie Dai; Xunwei Duan; Cuifang Wang; Xiaosi Lin; Jiaying Ke; Yixuan Wang; Xiaobo Zhang; Haipeng Liu

doi:10.1016/j.fsi.2019.08.009

miRNAs induced by white spot syndrome virus involve in immunity pathways in shrimp Litopenaeus vannamei

Fish Shellfish Immunol. 2019 Oct:93:743-751. doi: 10.1016/j.fsi.2019.08.009. Epub 2019 Aug 10.

Authors

Wenlin Wu¹, Congjie Dai¹, Xunwei Duan¹, Cuifang Wang¹, Xiaosi Lin¹, Jiaying Ke¹, Yixuan Wang¹, Xiaobo Zhang², Haipeng Liu³

Affiliations

¹ Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, 362000, China.
² Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou Normal University, Quanzhou, 362000, China; School of Life Science, Zhejiang University, Hangzhou, 310004, China. Electronic address: zxb0812@zju.edu.cn.
³ State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361102, Fujian, China; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources (Xiamen University), State-Province Joint Engineering Laboratory of Marine Bioproducts and Technology, Xiamen, 361102, Fujian, China. Electronic address: haipengliu@xmu.edu.cn.

PMID: 31408731
DOI: 10.1016/j.fsi.2019.08.009

Abstract

White shrimp Litopenaeus vannamei are widely cultured in the world and white spot syndrome virus (WSSV) led to huge economic losses in the shrimp industry every year. In the present study, miRNAs involved in the response of shrimp L. vannamei to WSSV infection were obtained through the Illumina HiSeq 2500 high-throughput next-generation sequencing technique. A total number of 7 known miRNAs and 54 putative novel miRNAs were obtained. Among them, 14 DEMs were identified in the shrimp infected with WSSV. The putative target genes of these DEMs were related to host immune response or signaling pathways, indicating the importance of miRNAs in shrimp against WSSV infection. The results will provide information for further research on shrimp response to virus infection and contribute to the development of new strategies for effective protection against WSSV infections.

Keywords: Immunity; Litopenaeus vannamei; WSSV; miRNAs.

MeSH terms

Animals
Immunity, Innate / genetics*
MicroRNAs / immunology*
Penaeidae / genetics*
Penaeidae / immunology*
White spot syndrome virus 1 / physiology*

Substances

MicroRNAs