Impairment of Th cell response in Card9 knockout mice with cutaneous mucormycosis caused by Rhizopus arrhizus

Exp Dermatol. 2019 Nov;28(11):1244-1251. doi: 10.1111/exd.14020. Epub 2019 Sep 4.

Abstract

Card9 is a signalling adaptor protein in the downstream of many innate pattern recognition receptors (PRRs) and exerts a significant role in antifungal immunity. To date, Card9 deficiency has been reported to be related to increased susceptibility to many fungal infections. In this study, we established mucormycosis murine model of Rhizopus arrhizus (R. arrhizus) using wild-type (WT) mice and Card9 knockout (Card9-/- ) mice to investigate the antifungal effect of Card9 against R. arrhizus infection. Card9-/- mice were more susceptible to R. arrhizus infection than WT mice, which could be related to the impaired NF-κB pathway activation, local cytokine production and Th cell responses in Card9-/- mice.

Keywords: Rhizopus arrhizus; Card9 knockout; Th cell response; cutaneous mucormycosis; mouse model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CARD Signaling Adaptor Proteins / physiology*
  • Cytokines / blood
  • Female
  • Host-Pathogen Interactions / immunology*
  • Male
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mucormycosis / immunology*
  • Mucormycosis / microbiology
  • Rhizopus / physiology*
  • T-Lymphocytes, Helper-Inducer / physiology*
  • Transcription Factor RelA / metabolism

Substances

  • CARD Signaling Adaptor Proteins
  • Card9 protein, mouse
  • Cytokines
  • Transcription Factor RelA