Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis

Methods Mol Biol. 2019:2038:223-237. doi: 10.1007/978-1-4939-9674-2_15.

Abstract

One key step in the activation of inducible transcription factors is their homooligomerization, which can be measured in individual living cells by a fluorescence microscopy technique called Number and Brightness analysis (N&B). In this chapter we describe how to acquire and analyze confocal microscopy time-series to provide information about transcription factor oligomerization in living cells using this technique.

Keywords: Image analysis; Live cell imaging; Number and brightness; Oligomerization; Transcription factor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Gene Expression Regulation
  • Humans
  • Microscopy, Confocal*
  • Molecular Imaging / methods*
  • Protein Multimerization
  • Single-Cell Analysis / methods*
  • Time Factors
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation

Substances

  • Transcription Factors