Spectrum-effect relationship of antioxidant and tyrosinase activity with Malus pumila flowers by UPLC-MS/MS and component knock-out method

Abstract

The active components of Malus pumila flowers on antioxidant and tyrosinase activity were investigated with the method of spectrum-effect relationship and knock-out. Some compounds were identified by UPLC-MS/MS method. The chemical fingerprints were established by HPLC and the activity of antioxidant and tyrosinase were assayed in vitro. Chromatographic peaks P34, P35, P39, P44, P45 and P49 were identified as phloridzin, hyperoside, astragalin, afzelin, quercetin and kaempferol by UPLC-MS/MS method. Hyperoside and kaempferol were identified in M. pumila flowers for the first time. When the concentration was 1 g/mL of sample (equivalent to raw material), the scavenging capacity of P35 (hyperoside) on DPPH free radicals were consistent with the spectrum-effect relationship. The scavenging capacity of P34 (phloridzin) and P45 (quercetin) on ABTS free radicals were consistent with the spectrum-effect relationship. The activation effect of P45 (quercetin) on tyrosinase was consistent with the spectrum-effect relationship. The inhibitory effect of P34 (phloridzin), P35 (hyperoside) on tyrosinase were consistent with the spectrum-effect relationship.

Keywords: Knock-out; Malus pumila flower; Spectrum-effect relationship.