Evaluating the genotoxic damage in bovine whole blood cells in vitro after exposure to thiacloprid

Abstract

Possible genotoxic effect of thiacloprid on bovine cultures of whole blood was investigated using chromosomal aberrations (CAs), micronuclei (MN), sister chromatid exchanges (SCEs), DNA damage and apoptotic DNA fragmentation assays. The cells of whole blood were exposed to thiacloprid (30, 60, 120, 240 and 480 μg mL^-1) for the last 24 and 48 h of cultivation. Thiacloprid did not induce significant increase in CAs after 24 and 48 h; only the concentration of 120 μg mL^-1 caused elevation of CAs (p < 0.05) after 24 h treatment. No clastogenic/aneugenic effect was observed by scoring of micronuclei. Considering replication damage reflected in SCEs, significant elevations were observed in both donors for 24 h (120-480 μg mL^-1; p < 0.01 or p < 0.05). In comet assay, statistically significant DNA damage was observed after 2 h exposure (240 and 480 μg mL^-1; p < 0.05, p < 0.01). DNA electrophoretic separation did not confirm the late apoptotic effect of thiacloprid. The decrease in additional variables such as mitotic index, cytochalasin-blocked proliferation and proliferation indices indicates the possible ability of thiacloprid to induce cytotoxic/cytostatic effects by affecting and/or inhibiting cell proliferation and to influence the cell cycle respectively.