Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays

Giulia Tomagra; Claudio Franchino; Alberto Pasquarelli; Emilio Carbone; Paolo Olivero; Valentina Carabelli; Federico Picollo

doi:10.1016/j.bpc.2019.106241

Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays

Biophys Chem. 2019 Oct:253:106241. doi: 10.1016/j.bpc.2019.106241. Epub 2019 Jul 31.

Authors

Giulia Tomagra¹, Claudio Franchino², Alberto Pasquarelli³, Emilio Carbone², Paolo Olivero⁴, Valentina Carabelli², Federico Picollo⁴

Affiliations

¹ Department of Drug and Science Technology, NIS Inter-departmental Centre, University of Torino, Corso Raffaello 30, 10125 Torino, Italy. Electronic address: giulia.tomagra@unito.it.
² Department of Drug and Science Technology, NIS Inter-departmental Centre, University of Torino, Corso Raffaello 30, 10125 Torino, Italy.
³ Institute of Electron Devices and Circuits, University of Ulm, 89069 Ulm, Germany.
⁴ Department of Physics, NIS Inter-departmental Centre, University of Torino, Italian Institute of Nuclear Physics, via Giuria 1, 10125 Torino, Italy.

PMID: 31398633
DOI: 10.1016/j.bpc.2019.106241

Abstract

Micro graphitic - diamond - multi electrode arrays (μG-D-MEAs) are suitable for measuring multisite quantal dopamine (DA) release from PC12 cells. Following cell stimulation with high extracellular KCl and electrode polarization at +650 mV, amperometric spikes are detected with a mean frequency of 0.60 ± 0.16 Hz. In each recording, simultaneous detection of secretory events is occurred in approximately 50% of the electrodes. Kinetic spike parameters and background noise are preserved among the different electrodes. Comparing the amperometric spikes recorder under control conditions with those recorders from PC12 cells previously incubated for 30 min with the dopamine precursor Levodopa (L-DOPA, 20 μM) it appears that the quantal size of amperometric spikes is increased by 250% and the half-time width (t_1/2) by over 120%. On the contrary, L-DOPA has no effect on the frequency of secretory events. Overall, these data demonstrate that the μG-D-MEAs represent a reliable bio-sensor to simultaneously monitor quantal exocytotic events from different cells and in perspective can be exploited as a drug-screening tool.

Keywords: Diamond MEA; Dopamine; L-Dopa; PC12 cells; Secretion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques*
Cells, Cultured
Diamond / chemistry*
Diamond / metabolism
Dopamine / chemistry
Dopamine / metabolism*
Electrodes
Graphite / chemistry*
Graphite / metabolism
PC12 Cells
Particle Size
Rats
Surface Properties

Substances

Diamond
Graphite
Dopamine