Abstract
Herein, we report a novel magnet-mediated antibody-boronate sandwich-typed assay (ABSTA) strategy for the ultrasensitive, specific, rapid, and enzyme-free detection of glycoproteins in complex samples. The proposed ABSTA method exhibited ultrahigh sensitivity for HCG with a detection limit of 0.19 mIU mL-1, which is approximately 40-fold lower than that of conventional sandwich enzyme immunoassay.
MeSH terms
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Antibodies, Immobilized / chemistry*
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Antibodies, Monoclonal / chemistry
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Boronic Acids / chemistry*
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Chorionic Gonadotropin / analysis
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Chorionic Gonadotropin / blood*
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Fluorescence
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Fluorescent Dyes / chemistry*
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Humans
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Immunoassay / methods
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Limit of Detection
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Magnets / chemistry*
Substances
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Antibodies, Immobilized
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Antibodies, Monoclonal
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Boronic Acids
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Chorionic Gonadotropin
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Fluorescent Dyes