In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells

Methods Mol Biol. 2019:2048:93-106. doi: 10.1007/978-1-4939-9728-2_11.

Abstract

In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.

Keywords: Hematopoietic progenitor cells; In vitro differentiation; Nonhuman primate-induced pluripotent stem cells; Preclinical model; T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD8-Positive T-Lymphocytes / physiology*
  • Cell Differentiation*
  • Cell Line
  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Coculture Techniques / instrumentation
  • Coculture Techniques / methods
  • Culture Media / metabolism
  • Cytokines / metabolism
  • Fibroblasts
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Fluorescent Antibody Technique, Direct / instrumentation
  • Fluorescent Antibody Technique, Direct / methods
  • Hematopoietic Stem Cells
  • Humans
  • Induced Pluripotent Stem Cells / physiology*
  • Mesenchymal Stem Cells
  • Mice
  • Primates

Substances

  • Culture Media
  • Cytokines