Abstract
In this chapter, we describe a protocol for hematopoietic differentiation of nonhuman primate (NHP)-induced pluripotent stem cells (iPSCs) derived from T cells and generation of T cells. Derivation of T cells from PSCs involves three steps: induction of PSCs to hematopoietic progenitor cells (HPCs), differentiation of HPCs into progenitor T cells, and maturation of progenitor T cells into mature T cells, in particular CD8 single-positive (SP) T cells.
Keywords:
Hematopoietic progenitor cells; In vitro differentiation; Nonhuman primate-induced pluripotent stem cells; Preclinical model; T cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD8-Positive T-Lymphocytes / physiology*
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Cell Differentiation*
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Cell Line
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Cell Separation / instrumentation
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Cell Separation / methods*
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Coculture Techniques / instrumentation
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Coculture Techniques / methods
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Culture Media / metabolism
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Cytokines / metabolism
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Fibroblasts
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Flow Cytometry / instrumentation
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Flow Cytometry / methods*
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Fluorescent Antibody Technique, Direct / instrumentation
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Fluorescent Antibody Technique, Direct / methods
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Hematopoietic Stem Cells
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Humans
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Induced Pluripotent Stem Cells / physiology*
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Mesenchymal Stem Cells
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Mice
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Primates