Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

Nicolas Kieffer; Laurent Poirel; Claudine Fournier; Brad Haltli; Russel Kerr; Patrice Nordmann

doi:10.3389/fmicb.2019.01673

Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

Front Microbiol. 2019 Jul 23:10:1673. doi: 10.3389/fmicb.2019.01673. eCollection 2019.

Authors

Nicolas Kieffer^{1

2}, Laurent Poirel^{1

2

3}, Claudine Fournier^{1

2}, Brad Haltli⁴, Russel Kerr⁴, Patrice Nordmann^{1

2

3

5}

Affiliations

¹ Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland.
² Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Fribourg, Switzerland.
³ Department of Medicine, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.
⁴ Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, PE, Canada.
⁵ Department of Medicine, Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland.

Abstract

The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.

Keywords: PAN-1; Pseudobacteriovorax antillogorgiicola; carbapenemase; environment; metallo-β-lactamase.