Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps

Vet Parasitol. 2019 Aug:272:58-63. doi: 10.1016/j.vetpar.2019.07.002. Epub 2019 Jul 12.

Abstract

Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnostic potentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previously reported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. multiceps.

Keywords: Antigen B; Coenurus cerebralis; Indirect ELISA; Metacestode; Taenia multiceps; Thioredoxin peroxidase.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antigens, Helminth / genetics*
  • Antigens, Helminth / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation
  • Peroxiredoxins / genetics*
  • Peroxiredoxins / metabolism*
  • Sensitivity and Specificity
  • Serologic Tests
  • Taenia / enzymology*
  • Taenia / metabolism
  • Taeniasis / diagnosis*
  • Taeniasis / parasitology

Substances

  • Antigens, Helminth
  • Peroxiredoxins