Abstract
In this study, we used LS174T cells as a model to investigate the protective effects of icariin and phosphorylated icariin on LPS-induced goblet cell dysfunction. Our results indicated that icariin and phosphorylated icariin increased the cell viability and decreased lactate dehydrogenase activity in LPS-treated LS174T cells. Icariin and phosphorylated icariin attenuated LPS-induced changes in mucin 2 synthesis and secretion. Besides, Icariin and phosphorylated icariin reduced the levels of ROS, MDA, and H2O2 and increased the activity of SOD, GPx, CAT, and T-AOC in LPS-treated LS174T cells. Moreover, the levels of IL-1β, IL-6, IL-8, and TNF-α were reduced in the Icariin and phosphorylated icariin group. Furthermore, Icariin and phosphorylated icariin decreased gene abundance or enzyme activity of Bip, XBP1, GRP78, CHOP, caspase-3, and caspase-4 in LPS-treated LS174T cells. Our data suggest that Icariin and phosphorylated icariin effectively attenuate LPS-induced intestinal goblet cell function damage through regulating oxidative stress, inflammation, apoptosis, and mucin expression.
Keywords:
Icariin; LPS; LS174T cells; mucus barrier; phosphorylated icariin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Apoptosis / genetics
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Caspase 3 / genetics
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Caspase 3 / metabolism
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Caspases, Initiator / genetics
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Caspases, Initiator / metabolism
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Catalase / metabolism
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Cell Proliferation / drug effects*
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Cell Survival / drug effects
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Endoplasmic Reticulum Chaperone BiP
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Flavonoids / metabolism
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Flavonoids / pharmacology*
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Glutathione Peroxidase / metabolism
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Goblet Cells / drug effects*
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Goblet Cells / enzymology
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Goblet Cells / metabolism
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism
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Humans
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Hydrogen Peroxide / metabolism
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Inflammation / genetics
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Inflammation / metabolism*
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Interleukin-1beta / metabolism
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Interleukin-6 / metabolism
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Interleukin-8 / metabolism
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L-Lactate Dehydrogenase / metabolism
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Lipopolysaccharides / pharmacology
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Malondialdehyde / metabolism
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Mucin-2 / biosynthesis
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Mucin-2 / metabolism
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Phosphorylation
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Reactive Oxygen Species / metabolism
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Superoxide Dismutase-1 / metabolism
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Transcription Factor CHOP / genetics
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Transcription Factor CHOP / metabolism
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Tumor Necrosis Factor-alpha / metabolism
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X-Box Binding Protein 1 / genetics
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X-Box Binding Protein 1 / metabolism
Substances
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DDIT3 protein, human
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Endoplasmic Reticulum Chaperone BiP
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Flavonoids
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Heat-Shock Proteins
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Interleukin-1beta
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Interleukin-6
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Interleukin-8
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Lipopolysaccharides
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Mucin-2
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Reactive Oxygen Species
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Tumor Necrosis Factor-alpha
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X-Box Binding Protein 1
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XBP1 protein, human
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Transcription Factor CHOP
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Malondialdehyde
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Hydrogen Peroxide
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L-Lactate Dehydrogenase
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Catalase
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Glutathione Peroxidase
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Superoxide Dismutase-1
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CASP4 protein, human
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Caspase 3
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Caspases, Initiator
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icariin