Identifying microbial community evolution in membrane bioreactors coupled with anaerobic side-stream reactor, packing carriers and ultrasonication for sludge reduction by linear discriminant analysis

Yue Zheng; Zhen Zhou; Xiaofang Ye; Jing Huang; Lingyan Jiang; Guang Chen; Liuyu Chen; Zhiwei Wang

doi:10.1016/j.biortech.2019.121920

Identifying microbial community evolution in membrane bioreactors coupled with anaerobic side-stream reactor, packing carriers and ultrasonication for sludge reduction by linear discriminant analysis

Bioresour Technol. 2019 Nov:291:121920. doi: 10.1016/j.biortech.2019.121920. Epub 2019 Jul 27.

Authors

Yue Zheng¹, Zhen Zhou², Xiaofang Ye¹, Jing Huang¹, Lingyan Jiang³, Guang Chen³, Liuyu Chen¹, Zhiwei Wang⁴

Affiliations

¹ Shanghai Engineering Research Center of Energy - Saving in Heat Exchange Systems, College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai 200090, China.
² Shanghai Engineering Research Center of Energy - Saving in Heat Exchange Systems, College of Environmental and Chemical Engineering, Shanghai University of Electric Power, Shanghai 200090, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address: zhouzhen@shiep.edu.cn.
³ Shanghai Chengtou Wastewater Treatment Co Ltd, Shanghai 201203, China.
⁴ State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.

PMID: 31382094
DOI: 10.1016/j.biortech.2019.121920

Abstract

An anoxic/oxic membrane bioreactor (AO-MBR), an anaerobic side-stream reactor (ASSR) coupled MBRs (A-MBRs), an A-MBR with carriers packed in ASSR (AP-MBR) and an AP-MBR with sludge ultrasonicated before ASSR (AUP-MBR) were operated for 261 d to investigate effects of ASSR, packing carriers and ultrasonication on sludge reduction and microbial population. Sludge reduction efficiencies of A-MBR, AP-MBR and AUP-MBR were 36.2%, 46.4% and 51.4%, respectively. Packing carriers and ultrasonication both enhanced hydrolysis by stimulating activities of α-glucosidase and protease, while uncoupling metabolism was enhanced greatly by packing carriers but slightly by ultrasonication. Linear discriminant analysis of effect size (LEfSe) results showed that packing carriers promoted the growth of hydrolytic and fermentative bacteria in bulk sludge, and enriched anaerobes and fermentative bacteria on the surface of carriers. Ultrasonication screened ultrasonication-resistant bacteria, and created an anaerobic environment beneficial to hydrolytic and fermentative bacteria.

Keywords: Anaerobic side-stream reactor; Carriers; Membrane bioreactor; Microbial community; Sludge reduction; Ultrasonication.

MeSH terms

Anaerobiosis
Bioreactors
Discriminant Analysis
Microbiota*
Rivers
Sewage*
Waste Disposal, Fluid

Substances

Sewage