Purpose: Glioma is a common and fatal intracranial tumor. Both miR-377 and lncRNA MEG3 are tumor suppressors. This study was performed to investigate the association between miR-377 and lncRNA MEG3 in glioma cells.
Methods: U118 and U251 cell lines were incubated in Dulbecco's modified Eagle's medium supplemented with miR-377 mimics, MEG3 siRNA (si-MEG3) and/or MEG3 overexpression plasmids (pc-MEG3) for 48 h. Cell migration, invasion, apoptosis, cell cycle distribution and the expression of E26 tansformation-specific-1 (ETS-1), phosphatase and tensin homologue (PTEN), E-cadherin, N-cadherin and β-catenin were detected.
Results: MiR-377 mimics increased MEG3 expression and decreased the number of migrated and invaded U118 and U251 cells, without influence on apoptosis in both cell lines. Si-MEG3 transfection increased U118 cell migration and invasion and rescued miR-377 mimics-induced inhibitory in cell migration and invasion. Si-MEG3 decreased U118 cell apoptosis and induced G0/G1 cell cycle arrest, and pc-MEG3 increased U251 cell apoptosis via arresting cell cycle at G2/M phage. MiR-377 mimics and si-MEG3 increased the relative expression level of N-cadherin mRNA, and both si-MEG3 and pc-MEG3 increased E-cadherin in glioma cells. MiR-377 mimics increased ETS-1 mRNA in U118 cells, but decreased it in U251 cells. PTEN was increased by miR-377 mimics and si-MEG3 and decreased by pc-MEG3 in glioma cells.
Conclusions: These results suggested the link interaction of MEG3 with miR-377 and PTEN, but not functioning as the competing endogenous RNA. MiR-377 mimics and MEG3 were tumor suppressors in glioma cells through regulating PTEN expression.
Keywords: Glioma; Invasion; Migration; lncRNA MEG3; microRNA-377.
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