Pretreatment of mulberry stem by cholinium-based amino acid ionic liquids for succinic acid fermentation and its application in poly(butylene) succinate production

Woranud Pakdeedachakiat; Nantharat Phruksaphithak; Apichat Boontawan

doi:10.1016/j.biortech.2019.121873

Pretreatment of mulberry stem by cholinium-based amino acid ionic liquids for succinic acid fermentation and its application in poly(butylene) succinate production

Bioresour Technol. 2019 Nov:291:121873. doi: 10.1016/j.biortech.2019.121873. Epub 2019 Jul 24.

Authors

Woranud Pakdeedachakiat¹, Nantharat Phruksaphithak², Apichat Boontawan³

Affiliations

¹ School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.
² Department of Chemistry, Faculty of Science, Thaksin University, 222 Moo 2, Baan Prao Sub-District, Papayom District, Phatthalung 93110, Thailand.
³ School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand; Center of Excellent in Agricultural Product Innovation, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand. Electronic address: apichat@sut.ac.th.

PMID: 31377506
DOI: 10.1016/j.biortech.2019.121873

Abstract

Cholinium-glycinate ([Ch][Gly]) and cholinium-alanate ([Ch][Ala]) were investigated on the pretreatment of mulberry stem (MS). It resulted in an increase of glucose from 14% to more than 74% compared to the untreated sample. Pretreatment by reused [Ch][Gly] showed good performance for delignification of >60%, and improved structural polysaccharide digestion. Each fractional component has high potential for lignin purification, and succinic acid fermentation. The extracted lignin with [Ch][Gly] showed >90% purity with good qualities of aromatic unit as confirmed by FT-IR and ¹H NMR spectra. The carbohydrate rich material was employed for succinic acid fermentation with the highest yield of succinic acid more than 0.89 g_{succinic acid}/g_glucose. After purification, poly(butylene) succinate (PBS) was synthesized, and was characterized in comparison to commercial PBS.

Keywords: Cellulolytic and xylanolytic enzyme; Ionic liquid; Lignocellulose; Poly(butylene) succinate; Succinic acid fermentation.

MeSH terms

Amino Acids / metabolism
Butylene Glycols / metabolism*
Fermentation*
Glucose / metabolism
Ionic Liquids / chemistry
Lignin / chemistry
Morus / metabolism*
Polymers / metabolism*
Succinic Acid / metabolism*

Substances

Amino Acids
Butylene Glycols
Ionic Liquids
Polymers
bionole
Lignin
Succinic Acid
Glucose