Silver nanoparticle exposure impairs ion regulation in zebrafish embryos

Abstract

The potential toxicity of silver nanoparticles (AgNPs) to the early stages of fish is still unclear. This study used zebrafish embryos as a model to investigate the toxic effects of AgNPs on ion regulation by skin ionocytes. Zebrafish embryos were exposed to AgNPs for 96 h (4-100 h post-fertilization (hpf)) or 4 h (96-100 hpf). After 96 h of exposure to 5 and 10 mg/L AgNPs, survival rates had decreased to 42% and 0%, respectively; the body length had also significantly decreased at 5 mg/L. Whole-body Na⁺ and K⁺ contents significantly decreased at 1 and 3 mg/L, while Ca²⁺ contents decreased at ≥0.1 mg/L. H⁺ secretion by the skin significantly decreased at 1 mg/L. The density of skin ionocytes labeled with rhodamine 123 (a mitochondrion marker) decreased by 25% and 55% at 1 and 3 mg/L, respectively; and 54% of ionocytes (at 3 mg/L) were deformed from an oval to a spinous shape. After 4 h of exposure to 1 and 5 mg/L, whole-body Na⁺ and Ca²⁺ contents, H⁺ secretion, and density of ionocytes had also significantly decreased. This study revealed the toxicity of AgNPs to skin ionocytes and ion regulation in the early stages of zebrafish embryos.

Keywords: Ion regulation; Ionocyte; Mitochondria; Rhodamine 123; SIET; Silver nanoparticles; Skin; Zebrafish.