Exploring the correlation between deltamethrin stress and Keap1-Nrf2-ARE pathway from Drosophila melanogaster RNASeq data

Genomics. 2020 Mar;112(2):1300-1308. doi: 10.1016/j.ygeno.2019.07.019. Epub 2019 Jul 31.

Abstract

Deltamethrin (DM) is widely used in a variety of pest control, resulting in serious drug resistance. Keap1-Nrf2-ARE is the antioxidant stress pathway. We identified 268 genes differentially expressed (DEGs) in Drosophila Kc cells treated with DM, including up-regulated 180 genes and down-regulated 88 genes compared with the control group (fold-change≥2, qValue≤0.001) by RNA-seq, they are mainly linked to metabolic process, stimulation response, immune system process. When the cells are treated with DM in the case of overexpression of the Keap1 gene, the cytochrome P450 family genes were significantly down-regulated, and some diseases-related genes and non-coding genes also changed. Our data shown that Keap1-Nrf2-ARE pathway may play an important role in DM stress, which will provide a new direction for studying the mechanism of insect resistance.

Keywords: Deltamethrin; Detoxification; Drosophila Kc cells; Keap1-Nrf2-ARE pathway; RNA-Seq.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidant Response Elements*
  • Apoptosis
  • Cell Line
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster
  • Insecticides / toxicity
  • Kelch-Like ECH-Associated Protein 1 / genetics
  • Kelch-Like ECH-Associated Protein 1 / metabolism*
  • Nitriles / toxicity
  • Pyrethrins / toxicity
  • RNA-Seq
  • Stress, Physiological*

Substances

  • Drosophila Proteins
  • Insecticides
  • Keap1 protein, Drosophila
  • Kelch-Like ECH-Associated Protein 1
  • Nitriles
  • Pyrethrins
  • decamethrin