Expression of Lhx6 in the adult and developing mouse retina

Daqiang He; Rui Guo; Dongwang Zheng; Ping Li; Xuhui Dong; Lin Gan

doi:10.1016/j.gep.2019.119065

Expression of Lhx6 in the adult and developing mouse retina

Gene Expr Patterns. 2019 Dec:34:119065. doi: 10.1016/j.gep.2019.119065. Epub 2019 Jul 31.

Authors

Daqiang He¹, Rui Guo², Dongwang Zheng³, Ping Li⁴, Xuhui Dong², Lin Gan⁵

Affiliations

¹ College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China; Institute of Life Sciences, Hangzhou Normal University, Hangzhou, 310018, Zhejiang, China; Department of Laboratory Medicine, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, 310006, Zhejiang, China.
² College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
³ Department of Reproductive Physiology, Zhejiang Academy of Medical Sciences, Hangzhou, 310000, Zhejiang, China.
⁴ HangZhou CalyGene Bitechnology Limited Company, Hangzhou, 310013, Zhejiang, China.
⁵ College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China; Institute of Life Sciences, Hangzhou Normal University, Hangzhou, 310018, Zhejiang, China. Electronic address: lin_gan@idrbio.org.

PMID: 31376504
DOI: 10.1016/j.gep.2019.119065

Abstract

Purpose: To investigate the expression patterns of LIM Homeobox 6 (Lhx6) in the adult and developing mouse retina.

Methods: The Lhx6-GFP knock-in allele was used to activate constitutive expression of a GFP reporter in Lhx6 expressing cells. Double labeling with GFP and retinal markers in the mouse retina at postnatal day 56 (P56) was performed to identify the cell types expressing Lhx6. To determine the neuronal cell types that express Lhx6, double labeling with GFP and various retinal markers was employed in the differentiating retina at P7 and P15.

Results: GFP + Lhx6 lineage cells were determined in Brn3a + retinal ganglion cells (RGCs), ChAT + amacrine cells (ACs), and Islet-class LIM-homeodomain 1 (Isl1+) ACs in the mouse retina at P56. In the ganglion cell layer (GCL), Lhx6 was expressed in Brn3a + RGCs but not Brn3b + RGCs at P15. Moreover, in the inner nuclear layer (INL), Lhx6 was not expressed in Bhlhb5+ ACs at P15. However, Lhx6 was weakly expressed in Glyt1+ ACs and Pax6+ ACs, and strongly expressed in Isl1+ and ChAT + ACs at P15.

Conclusion: Lhx6 was expressed in RGCs and ACs in both the adult and developing mouse retina.

Keywords: ACs; Expression; Lhx6; RGCs; Retina.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Age Factors
Amacrine Cells / metabolism
Animals
Cell Lineage
DNA-Binding Proteins / metabolism
Female
Gene Expression Regulation, Developmental / genetics
LIM-Homeodomain Proteins / genetics*
LIM-Homeodomain Proteins / metabolism
Male
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins / genetics*
Nerve Tissue Proteins / metabolism
Retina / growth & development*
Retina / metabolism*
Retinal Ganglion Cells / metabolism
Transcription Factors / genetics*
Transcription Factors / metabolism
Transcriptome / genetics

Substances

DNA-Binding Proteins
LHX6 protein, mouse
LIM-Homeodomain Proteins
Nerve Tissue Proteins
Transcription Factors