ISCO using activated sodium persulphate is a widely used technology for treating chlorinated solvent source zones. In sensitive areas, however, high groundwater sulphate concentrations following treatment may be a drawback. In situ biogeochemical transformation, a technology that degrades contaminants via reduced iron minerals formed by microbial activity, offers a potential solution for such sites, the bioreduction of sulphate and production of iron sulphides that abiotically degrade chlorinated ethenes acting as a secondary technology following ISCO. This study assesses this approach in the field using hydrochemical and molecular tools, solid phase analysis and geochemical modelling. Following a neutralisation and bioaugmentation, favourable conditions for iron- and sulphate-reducers were created, resulting in a remarkable increase in their relative abundance. The abundance of dechlorinating bacteria (Dehalococcoides mccartyi, Dehalobacter sp. and Desulfitobacterium spp.) remained low throughout this process. The activity of iron- and sulphate-reducers was further stimulated through application of magnetite plus starch and microiron plus starch, resulting in an increase in ferrous iron concentration (from <LOQ to 337 mg/l), a decrease in sulphate concentration by 74-95% and production of hydrogen sulphide (from <LOQ to 25.9 mg/l). At the same time, a gradual revival of dechlorinators and an increase in ethene concentration was also observed. Tetrachloroethene and trichloroethene concentrations decreased by 98.5-99.98% and 75.4-98.5%, respectively. A decline in chlorine number indicated that biological dechlorination contributed to CVOC removal. This study brings new insights into biogeochemical processes that, when properly engineered, could provide a viable solution for secondary treatment.
Keywords: Biogeochemical transformation; Chlorinated solvents; Geochemical modelling; Indigenous microorganisms; Molecular tools; Solid phase analysis.
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