Caution: Plasmid DNA topology affects luciferase assay reproducibility and outcomes

Biotechniques. 2019 Sep;67(3):94-96. doi: 10.2144/btn-2019-0060. Epub 2019 Aug 1.

Authors

Emma Tudini^{1

2}, Lez J Burke¹, Phillip J Whiley^{1

2}, Jan Sevcik^{1

3}, Amanda B Spurdle², Melissa A Brown¹

Affiliations

¹ School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, 4067 QLD, Australia.
² Department of Genetics & Computational Biology, QIMR Berghofer Medical Research Institute, Herston, 4006 QLD, Australia.
³ Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic.

PMID: 31368777
DOI: 10.2144/btn-2019-0060

No abstract available

Keywords: assays; cell culture; gene expression analysis; in vitro models; transfection.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

BRCA1 Protein / genetics
DNA / analysis*
DNA / genetics
Genes, Reporter
Humans
Luciferases / analysis*
Luciferases / genetics
Luminescent Measurements* / methods
MCF-7 Cells
Plasmids / analysis*
Plasmids / genetics
Reproducibility of Results
Transfection* / methods

Substances

BRCA1 Protein
DNA
Luciferases