Microalgal assimilation of vitamin B12 toward the production of a superfood

Abstract

A network of components from different metabolic pathways is the building scaffold of an indispensable compound in the human organism-vitamin B₁₂ . The biosynthesis of this compound is restricted to a limited number of representatives of bacteria and archaea, while vitamin B₁₂ -dependent enzymes are spread through several domains of life. Different attempts have been performed to increase vitamin B₁₂ levels in dietary products, particularly in vegetarian and vegan dietary regimes. The integration of vitamin B₁₂ in microalgae through symbiosis with microorganisms generally recognized as safe, for example the probiotic Lactobacillus reuteri, can even increase the nutritional value of the microalgal biomass. This study reviews the microbial production of vitamin B₁₂ based on genetic analyses and chemical studies. Recent genetic approaches are focused, particularly potential metabolic engineering targets to increase vitamin B₁₂ production. The bioincorporation of vitamin B₁₂ in microalgae as an attempt to provide a superfood is also reviewed. PRACTICAL APPLICATIONS: Novel food habits (i.e., vegan lifestyle) may lack relevant nutrients, including vitamin B₁₂ . Therefore, there is an increased demand for dietary products rich in vitamin B₁₂ . Of potential interest is the provision of microbial-based superfood rich in numerous nutrients, including this vitamin. This manuscript provides an in-depth and timely overview on vitamin B₁₂ biosynthesis and the major advances on metabolic engineering for improved vitamin B₁₂ production by probiotic bacteria and other microorganisms generally recognized as safe. A relevant advance would result from the bioincorporation of vitamin B₁₂ in alternative microorganisms (non-vitamin B₁₂ producers) increasingly recognized as superfood, that is microalgae.

Keywords: bacteria-microalgae symbiosis; human nutrition; superfood; vegan lifestyle; vitamin B12.