Abstract
The fluorinase enzyme from Streptomyces cattleya is shown to catalyse a direct displacement of bromide and iodide by fluoride ion from 5'-bromodeoxyadenosine (5'-BrDA) and 5'-iododeoxyadenosine (5'-IDA) respectively to form 5'-fluorodeoxyadenosine (5'-FDA) in the absence of l-methionine (l-Met) or S-adenosyl-l-methionine (SAM). 5'-BrDA is the most efficient substrate for this enzyme catalysed Finkelstein reaction.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Bacterial Proteins / chemistry*
-
Catalysis
-
Deoxyadenosines / chemistry*
-
Halogens / chemistry*
-
Kinetics
-
Methionine / chemistry
-
Molecular Conformation
-
Oxidoreductases / chemistry*
-
S-Adenosylmethionine / chemistry
-
Streptomyces / enzymology
-
Thermodynamics
Substances
-
Bacterial Proteins
-
Deoxyadenosines
-
Halogens
-
S-Adenosylmethionine
-
Methionine
-
Oxidoreductases
-
fluorinase
-
2'-deoxyadenosine