A ratiometric fluorescent DNA nanoprobe for cerebral adenosine triphosphate assay

Zhichao Liu; Peicong Wu; Yaoyao Yin; Yang Tian

doi:10.1039/c9cc05046a

A ratiometric fluorescent DNA nanoprobe for cerebral adenosine triphosphate assay

Chem Commun (Camb). 2019 Aug 28;55(67):9955-9958. doi: 10.1039/c9cc05046a. Epub 2019 Jul 31.

Authors

Zhichao Liu¹, Peicong Wu¹, Yaoyao Yin¹, Yang Tian¹

Affiliation

¹ Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, P. R. China. ytian@chem.ecnu.edu.cn.

PMID: 31364619
DOI: 10.1039/c9cc05046a

Abstract

A silver nanocluster-based ratiometric fluorescent nanosensor was developed for the determination of ATP in the cerebrospinal fluid of a mouse brain. Using this useful tool with good stability and high selectivity as well as a wide linear detection range, it was found that the ATP concentration in a mouse brain with Alzheimer's disease was 2300-fold higher than that in a normal one.

MeSH terms

Adenosine Triphosphate / cerebrospinal fluid*
Alzheimer Disease / metabolism
Alzheimer Disease / pathology
Animals
Brain / cytology
Brain / pathology
Brain Chemistry*
Cerebral Cortex / chemistry
DNA / chemistry*
Fluorescent Dyes / chemistry*
Hippocampus / chemistry
Metal Nanoparticles / chemistry*
Mice
Nucleic Acid Conformation
Silver / chemistry
Spectrometry, Fluorescence / methods

Substances

Fluorescent Dyes
Silver
Adenosine Triphosphate
DNA