The diversity of fungi allows for their colonisation in different environments, including wood destined for power generation, with an ability to degrade or hinder its use. Torrefaction or pre-carbonisation, a low oxygenation heat treatment with temperatures between 200 and 300 °C, accumulates carbon and lignin, decreases hygroscopicity, increases energy efficiency and reduces the wood attractiveness to xylophagous microorganisms. This work aimed to study the resistance of Eucalyptus urophylla wood chips, submitted to torrefaction temperatures of 180, 220 and 260 °C for 20 minutes, to xylophagous fungi, according to the ASTM D-2017 method (2005). The white rot fungi Phanerochaete chrysosporium, Pleurotus ostreatus and Trametes versicolor and the brown rot fungus Gloeophyllum trabeum were used. After 12 weeks of exposure, the mass losses of wood samples in natura and torrified at 180 °C attacked by Pleurotus ostreatus and Trametes versicolor was higher. Torrefaction increased the resistance to fungi; the treatment at 260 °C was the most efficient with lower mass losses caused by fungi attacks and, consequently, greater resistance to the fungi tested.