A selective fluorescent turn-on probe for imaging peroxynitrite in living cells and drug-damaged liver tissues

Lili Xia; Yao Tong; Lushen Li; Mengyuan Cui; Yueqing Gu; Peng Wang

doi:10.1016/j.talanta.2019.06.032

A selective fluorescent turn-on probe for imaging peroxynitrite in living cells and drug-damaged liver tissues

Talanta. 2019 Nov 1:204:431-437. doi: 10.1016/j.talanta.2019.06.032. Epub 2019 Jun 11.

Authors

Lili Xia¹, Yao Tong¹, Lushen Li², Mengyuan Cui¹, Yueqing Gu¹, Peng Wang³

Affiliations

¹ Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China.
² Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.
³ Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: wangpeng@cpu.edu.cn.

PMID: 31357316
DOI: 10.1016/j.talanta.2019.06.032

Abstract

Peroxynitrite anion (ONOO^-), one of the reactive nitrogen species (RNS), plays momentous roles in physiological and pathological processes especially in a range of oxidative stress-related diseases. Moreover, abundant ONOO^- is generated in the liver tissues of drug-induced liver injury. We report herein a novel small molecule fluorescent probe KC-ONOO for monitoring ONOO^- based on boronate. The probe displayed high sensitivity and good selectivity towards ONOO^-. A good linear relationship was observed between the fluorescent intensity at 530 nm and the concentration of ONOO^- ranged 0-10 μM with a detection limit of 1.5 × 10^-8 M. Furthermore, our probe was successfully applied for imaging ONOO^- in living cells and drug-damaged liver tissues with low cytotoxicity, demonstrating the probe KC-ONOO has great potential to further elucidate more biological roles of ONOO^-.

Keywords: Drug-induced liver injury; Fluorescent probe; Imaging; Living cells; Peroxynitrite.

MeSH terms

Animals
Benzothiazoles / chemical synthesis
Benzothiazoles / chemistry*
Benzothiazoles / toxicity
Boronic Acids / chemical synthesis
Boronic Acids / chemistry*
Boronic Acids / toxicity
Chemical and Drug Induced Liver Injury / metabolism
Chemical and Drug Induced Liver Injury / pathology
Coumarins / chemical synthesis
Coumarins / chemistry*
Coumarins / toxicity
Fluorescence
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Fluorescent Dyes / toxicity
Hep G2 Cells
Humans
Limit of Detection
Liver / pathology
Male
Mice
Microscopy, Confocal / methods
Microscopy, Fluorescence / methods
Peroxynitrous Acid / analysis*
Peroxynitrous Acid / chemistry

Substances

Benzothiazoles
Boronic Acids
Coumarins
Fluorescent Dyes
Peroxynitrous Acid